Dopamine Neuron Characteristics

Dopamine Neuron CharacteristicsSaid Mohammed Al HakmaniThe question of this assignmentDiscuss the characteristics of a dopamine neuron and how does this relate to what has been achieved in the culture dish.IntroductionIn our body a lot of organs that possess our utilization of movement and control. Parkinsons disease extend to our move. It happen when there is a problem of the certain of nervous cellphones in the brain. The nerve cells make an important chemical is named dopamine. The dopamine sends signals to the kick downstairs of the brain that controls movement. Also, the dopamine lets the move of our muscle smoothly. The Parkinsons disease breakdown the nerve cells that make the dopamine. When the person do not deliver enough dopamine the problem of move can start and the person cannot control the way where to move. (1)Figure 1 shows the nerve cells in the brain (1)The Parkinsons disease is progressive, which means it gets worse over time. But this happen slowly some ye ars. Until now no one know what acutely makes the nerve cells breakdown. However, scientists ar doing a lot of research to know the reason of the cells breakdown. Some study say Parkinson disease is gene ab radiation pattern in some people only if so far there is enough proof to show that its always inherited.(1)Braaks hypothesis proposes that the Parkinsons disease begins in the digestive tract and in the brains centre of smell. The theory is supported by the fact that symptoms associated with digestion and smell happen very early on the disease. Professor Jia- Yi Lis research team has now been able to tack this process further, from the gut to the brain in rat models. The experiment shows how the toxic protein alpha-synculein is transferee from one cell to another in the beginning celestial orbiting the brains movement centre, giving increase to the characteristic of movement in the Parkinsons disease. He prove that disease process really can travel from the peripheral nervou s sy basis to the central nervous sy infrastructure, in this case from the wall of the gut to the brain.(3)Usually people start to accommodate symptoms of Parkinsons disease between the ages of 50 and 60. But in certain case start earlier.The symptoms of Parkinsons disease first may be get word is fear which means shaking or trembling. And these may affect the hands, arms, or legs. It is one of the most common signs yet not everyone has it. Also, not everyone with a tremor has Parkinsons disease. The tremor often starts only one side of the body or just one arm or leg. In addition, Parkinsons affects the arch muscles all through the body, so it causes problems like trouble swallowing or constipation. In the later stages of Parkinsons disease a person may have inflexible expression trouble speaking and other problems. Some people lose mental skills. (1)A dopamineDopamine is a chemical signal that transfers information from one neuron and the next neuron. It released the signals from the first neuron and it floats into the synapse (the space between the two neurons) to the second neuron. The receptors bumps the signals down to the receiving neurons. The dopamine interested in many distinct important pathways and it known as the mesolimbic pathways which starts with cells in the ventral tegmental area in the midbrain.(2) The dopamine plays numbers of roles in human and other animals. It controls many functions such as movement, memory, pleasurable reward, attention, sleep, learning, mood, inhibition of prolactin production, and behaviour. The increase and deficiency of this dopamine chemical is cause of several disease like Parkinson disease. In the brain, part called the basal ganglia regulates movement. meanspirited ganglia in enchantment depend on a certain amount of dopamine to function. The action of this occurs by dopamine receptors D 1-5. The deficiency in dopamine in the brain may pay back delayed and uncoordinated the movement and other functio n of the body. (3)The dopamine is important neurotransmitter in the brain substantial part of the overall dopamine in the body is produced outside the brain by mesenteric organs. The production of dopamine within the central nervous sy root, depend on the dopamine biosynthesis. There are two steps of dopamine biosynthesis takes place in the cytosol of CAergic neurons and starts with hydroxylase of L-tyrosine at the phenol ring by tyrosine hydroxylase to produce DOPA.(4)al-Qaeda cellThe stem cell have potential to develop into many different cell types in the body during early life and trainth. In many tissues they serve as a sort of internal repair system, divided without limit to replace other cells as long as the person or animal is still alive. When a stem cell divides, each new cell has the ability either to remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell and other cells.(5)The stem cel ls are featured from other cell types by two important characteristics. First, they are capable of variation themselves through cell division, sometimes after long periods of inactivity. Second, under some physiologic or experimental conditions, stem cell can be become tissue- or organ-specific cells with special functions. In certain organs, such as the gut and bone marrow, they regularly divide to repair and replace worn out or change tissues. In addition, in other organs, such as the pancreas and the heart, stem cells only divide under special conditions. (5)Scientists primarily worked with two types of stem cells from animals and serviceman embryotic stem cells and non- immature somatic or adult stem cells.In 1981 Scientists discovered ways to derive embryotic stem cells from early mouse embryos virtually 30 years ago. In 1998 the detailed study of the biology of mouse stem cells led to the discovery. After that method to derive stem cells from human embryos and grow the cel ls in the laboratory. These cells are called human embryotic stem cells. The embryos used to studies were created for reproductive purposes through in vitro fertilization procedures. When they were no longer fatalityed for that purpose, they were donated for research with the cognizant consent of the donor. Researchers made another breakthrough by identifying conditions that would allow some specialized adult cells to be reprogrammed to assume a stem cell-like kingdom in 2006. This new kind of stem cell, called induced pluripotent stem cells (iPSCs). (5) The Stem cells are important for living organisms for different reasons. In the three to five days old embryo, called a blastocyst, the inner cells give rise to the entire body of the organism, include all of the many specialized cell types and organs such as the, lungs, heart, skin and other tissues. In certain adult tissues, such as bone marrow, brain, and muscle, the adult stem cells generate replacements for cells that are lost through normal injury, or disease. The stem cells make new potentials for treating diseases such as heart disease and Parkinson disease. However, much work remains to be through in the laboratory to understand how to use these cells therapies to treat disease.(5) Research on stem cells continues to advance about how an organism develops from a single cell and how healthy cells replace damaged cells in adult organisms. The stem cell research is one of the fascinating areas in contemporary biology.(5)The laboratory of human embryonic stem cellThe communications protocol of the laboratory that culture the stem cells must contain special equipment use to avoiding the contamination during make the HESC culture. The equipment that are use a CO2 incubator, that cannot use the purifying to clean use 70% of ethanol to get the best result to control the contamination. Used a biocontainment hood to control of microorganisms. A microscope with phase wrinkle optics to see the cell and col ony morphology. A refrigerator 20 and freezers 80.Use the liquid nitrogen freezer to store the stocks of HESC and feeders. Also, need small water bathtub 37 to thawing the cell that are frozen.(6)Feeder cultureThe feeder culture use to growth of HESC. It prepare primary fibroblast that come from the CF-1 mice. The CF-1 mouse embryonic fibroblasts give the optimal growth of HESC without differentiation and recommended for many reason such as function well making feeder, available and cheaper. (6)The methods culture HESCThe first subject need to culture the HESC is good feeder. Human embryonic stem cell should be passaged when the colonies reach size 300 to 500 cells. The plate should be passaged big(p) culture of colonies. The cell always passaged to the plate of feeder cell less than seven days. (6)The materials that required for passaging Human embryonic stem cell are ESC medium, 15 ml centrifuge tubes, calcium/magnesium free PBS, collagenase type IV, cell scraper ,10 cm plates and 0.1% gelatin.(6)The procedureTable show the methods of passaging HESC adopted steps only but I create the table (6)Growth of the HESCTo growth the human embryonic stem cell transfer cell from preimplantation stage embryo to the culture dish. The cell will grow on the culture dish and will spread and divided on the surface of the media. The culture dish contain the mouse embryonic cell which provide the nutrients into the culture media to growth the cell. However, researcher now devised to grow the embryonic stem cells without mouse feeder cells to avoid the risk viruses that can transfer from the media to the human. (5)The test use to identify embryonic stem cells in the laboratory. There is no standard test approved yet to the scientists who study human embryonic stem cells. However, the laboratories that grow the human embryonic stem cells use different types of test such asGrowing and subculture of the stem cell for many months to make sure the health of the cell.Use techniq ues to determine the presence of transcription factors that produced by undifferentiated cells. These are two important transcription factors Nanog and Oct-4. The both factors help the genes to turn on and off to make maintain the stem cell to undifferentiated and self-renewal.(5)The GMPThere are many challenges to use the stem cells for replacement therapy. However, the good manufacturing entrust (GMP) is quality assurance system that use to make sure the product of stem cells is good and safe to use for treatment. The GMP in human embryonic stem cell culture required several components for good clinical practice. First, the culture or the feeder media that use in the laboratory must be reach to standard of GMP. Second, the inner cell mass of the blastocyst and passaging of the cell. Final, all components of the culture and cryopreservation media should validated to the GMP system. (7)The ethical issue surrounding the use of embryonic cell need be carefully controlled. In UK human embryonic stem cell can collected from left over embryos produced as part of vitro fertilisation. This can done under strict UK guidelines from Human Fertilisation Embryology Authority and only in the early stage embryos can be use in research up to maximum of 14 day of development.(8)The power of stem cellIn 1980, the researcher take stem cell inside the developing embryos and growth in the laboratory to make the human embryonic stem cell. However, the adult stem cell are extremely powerful and easier for researcher to make embryonic stem cell and can converted into any type of cell in the body. But there is risk that the stem cell may grow uncontrollable in the body which cause to formation of tumour.(8)Scientist in Japan worked to make stem cell from normal adult cell in 2006 and this called induced pluripotent stem cell (iPS). This iPS cell in the future may use to make for the longanimous own cell and it be safe from other factor the affect the patient. The researcher attemp ted to treat the cell that are die from the nervous cell by make experiment on rat and mice. It show progression to develop of dopamine that make replace of nervous cell. The researcher can measure the increase of dopamine neuron function in striatum of the patient by using positron emission tomography that make image of the brain.(5)Many researchers believe that embryonic stem cell could be excellent tooth root of dopamine neurons because can grow in culture dish and make into any type of cell after long time in culture. Now, the dopamine neurons have been generated from the Human embryonic stem cell. One study research use the special type of companion cell with specific growth factors to get along the differentiation of the embryonic stem cell through several stages into dopamine (5)ReferencesWebmd.com, (2014). Parkinsons Disease Center Symptoms, Treatments, Causes, Tests, Diagnosis, and Prognosis. online Available at http//www.webmd.com/parkinsons-disease Accessed 15 Nov. 201 4.Slate cartridge clip, (2014). Slate Magazine Politics, Business, Technology, and the Arts. online Available at http//www.slate.com Accessed 16 Nov. 2014.News-Medical.net, (2014). Health News and Information News Medical. online Available at http//www.news-medical.net Accessed 16 Nov. 2014.Meiser, J., Weindl, D. and Hiller, K. (2013). Complexity of dopamine metabolism. kiosk Commun Signal, 11(1), p.34.Stemcells.nih.gov, (2014). NIH Stem Cell Information Home Page. online Available at http//stemcells.nih.gov Accessed 16 Nov. 2014.Stemcell.umn.edu, (2014). Home MED Stem Cell Institute, University of Minnesota. online Available at http//www.stemcell.umn.edu Accessed 20 Nov. 2014.Unger, C., Skottman, H., Blomberg, P., Sirac Dilber, M. and Hovatta, O. (2008). Good manufacturing practice and clinical-grade human embryonic stem cell lines. Human Molecular Genetics, 17(R1), pp.R48-R53.Stem cells the story so far. (2014). progress the research magazine of Parkinsons UK, (15 summer).